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Respiratory suppression (although this is typically associated with much higher doses of phenobarbital than 20 mg/kg)

To prepare 10 mM HCl: Take 1 mL of standard concentrated HCl (12 M) Add to 1,199 mL of sterile water yields 10 mM HCl Alternatively, purchase pre-prepared sterile 10 mM HCl or use a validated dilution from a laboratory stock solution Alternative Solvent: 1% Acetic Acid in Sterile Water Some protocols use 1% acetic acid (glacial acetic acid diluted in sterile water) as the initial reconstitution vehicle

Link Gulak MA, Murphy P

doi: 10.1111/j.1365-2621.1947.tb16401.x 112 Kalantar-ZadehKBereanKJBurgellREMuirJGGibsonPR

Diabetes 61, 364371

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