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The RGLs undergo asymmetrical cell division and generate one RGL and either an astroglia or a transiently amplifying intermediate neural progenitor cell (IPC
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The luminescence of the supernatant was assayed by a luminometer (Berthold Detection System, Pforzheim, Germany)
For the DDD -Ube2e2-COP1-c-Jun-STK40 complex, untagged DDB1 and DDA1, TSF-tagged human DET1 and STK40, N-terminal GST- and C-terminal His-tagged COP1, MBP-tagged Ube2e2 and c-Jun were co-transfected into Expi293F cells
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