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The supernatant was loaded onto a 1 mL Nickel-Sepharose resin column and washed with 30 column volumes (CV) of washing buffer (50 mM TrisHCl pH 8.0, 300 mM NaCl and 20 mM imidazole)
Phase-2 : Partially depleted toxins of phase-1 are neutralised by enzymes
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Features suggestive of diastasis recti include: A generalised abdominal bulge, often more noticeable when sitting up or straining A feeling of reduced core strength A visible or palpable gap in the middle of the abdomen The bulge is not confined to the C-section scar area Diastasis recti contributes to abdominal prominence but does not, on its own, cause a localised shelf at the scar
Mahfouz R, El-Rewini H, Ghoneim SI, Sheta A, Ali EA, Ibrahim M
Nonaka, M
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