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To confirm that the PAT activity was responsible for the mislocalization of substrates in the SwissKASH assay, we generated zDHHC-inactive constructs by a Cys-to-Ser mutation in the catalytic DHHC domain 36
Anything that is going to be delivered through that channel has to be applied separately, either before the procedure (to be pulled through during stamping) or after (to diffuse through the closing channels)
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