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Lipid peroxidation was measured with using Bodipy 581/591 C11 (Fisher Scientific D3861) at 1 M following the manufacturers protocol

After 28 days, discard any remaining solution regardless of appearance

These findings are further supported by Chen et al

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The CagriSema metabolic preservation mechanism, specifically the maintenance of mitochondrial function in skeletal muscle during weight loss, could theoretically slow the rate of weight regain by preventing some of the metabolic adaptation that makes regain almost inevitable

8 Clinical positioning and pathways: risk-stratified decision matrix 8.1 Bridging gap between evidence and guidelines Traditional stepped care for PCOS-related infertility typically positions lifestyle intervention as the first-line therapy, followed by ovulation induction (OI) with letrozole or clomiphene, and ultimately ART