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Evolved variants displayed activity on GD_SS2 (Extended Data Fig

The DAC version uses a Drug Affinity Complex that binds albumin and extends half-life for a sustained GH and IGF-1 signal

These treatments are often categorized as elective or wellness therapies rather than medically necessary procedures

However, actual out-of-pocket costs depend on insurance coverage, pharmacy, and available savings programs

The new supernatant (cytosolic fraction) was directly prepared for SDS-PAGE, and the pellet (membrane fraction) was gently washed with hypo-osmotic buffer and resuspended in GST-lysis buffer supplemented with 0.5% SDS and protease inhibitors