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Since then, a number of preclinical studies have examined its possible impacts on inflammation, gastrointestinal health, and tissue repair

Hepatic 25-hydroxylation via cytochrome P450 isoform CYP2R1 converts cholecalciferol to 25(OH)D, followed by renal 1-hydroxylation through CYP27B1 to yield bioactive 1,25(OH)2D [153]

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cDNA was synthesized from 1 g RNA using a fPrime-Script RT Reagent Kit with gDNAEraser (Takara, Japan)