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In B10.RIII mice immunized with interphotoreceptor retinoid-binding protein 161-180 (IRBP161-180), gut microbiota composition was analyzed on day 7 (pre-onset), day 14 (peak onset), and day 21 (chronic phase)

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ApoA-IV is secreted only by the small intestine in humans (although it is expressed in the hypothalamus) and its synthesis in the gut is stimulated by active lipid absorption

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In a clean result you want one dominant peak (or multiple dominant peaks for blends), which shows the sample is almost entirely the intended peptide