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A large number of studies have concluded that the decreased intracellular pH of endosomes and lysosomes in tumor cells may assist in metastasis by activating proteases [42, 48]
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Endosomal pH investigation The effect of pH on FITC-agonist fluorescence was determined as fluorescence intensities [485 nm excitation, 520 nm emission (cut-off 495 nm)] of agonist solutions at specific pHs and data fitted using linear regression
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Aged WT and Glyco Hi mice were administered D 2 O for 14 days, a time point determined from prior experiments that allows for the capture of both short-lived and intermediate-lived proteins
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