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Heres a chart showing the glucoraphanin concentration by plant age: Increasing the bioavailability of sulforaphane in cooked broccoli: Adding mustard seeds (ground brown mustard seed) increased the availability of sulforaphane from cooked broccoli by 4-fold.[ref] The study used 1g of powdered mustard seed, which would be about a half teaspoon

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The acidity or alkalinity of the solution can impact the peptide's structure

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