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Besides importing from the extracellular environment, cells can synthesize serine from intracellular glucose through the de novo serine synthesis pathway (SSP), which is considered necessary for cancer cells (Locasale, 2013)

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AIChE J 22:10791090 Li Y, Chen J, Mao YY, Lun SY, Koo YM (1998) Effect of additives and fed-batch culture strategies on the production of glutathione by recombinant Escherichia coli

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48 h after transfection, cells were washed twice with pre-warmed PBS and fixed with 4% paraformaldehyde (PFA) for 1015 min