Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]
Dosing & Reconstitution Guide Educational guide for reconstitution and weekly dosing Standard / Gradual Approach (1 mL = ~5.0 mg/mL) Frequency: Inject once weekly subcutaneously [1] [2]
Acute episodes due to SPCD are often preceded by metabolic stress such as extended fasting, infections or vomiting
Injection is weekly, on the same day each week
However, with the steep price, it is hard to justify
There was an early study a couple of years ago that indicated a possible link to thyroid cancer, but that study had some limitations, and subsequent studies have shown that there is likely not a link there