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In 2016 Alexopoulou et al ., 1 demonstrated that Lewy Bodies contain predominantly K63-linked ubiquitin chains and that the quantity of these conjugates was inversely corelated with the localisation of Ubiquitin-specific protease 8 (USP8), a deubiquitinating enzyme (DUB) from the Ubiquitin-specific protease family

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In the above technical solution, in step 5, the freeze-drying comprises the following steps, preparing liquid and filling at 20-30 , putting a sample into a freeze dryer, quickly reducing the temperature of a plate layer to-40 , preserving heat for 1h, when the temperature of a partition plate is-10 +/-2 , preserving heat for 1h, quickly reducing the temperature of the plate layer to-40 within 1h, preserving heat for 3h, transferring to a cold trap at-50 , vacuumizing to below 10Pa, firstly preserving the temperature of the plate layer at-5 for 3h, then heating the plate layer to 10 within 3h, when a product is at 0 , starting to increase the temperature of the plate layer to 40 , and when the product is increased to 30 , preserving heat for 5 h

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