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Peroxynitrite (ONOO - ,0.1 M stock solution in 0.3 M NaOH-NaCl) was diluted to less than 1/100 with ice-cold water, just prior to quick exposure to HGF in PBS by vortexing at 1:2000 (see Figure 1a for the experimental design) 32
The peptides themselves need to be sourced properly and undergo vigorous testing for purity both in synthesis and in final preparation
Preclinical studies show minimal toxicity in research models, though some reports note temporary fatigue, nausea, or mild injection-site irritation
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Cannell, J
(2007) Hepatology 45:1118
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