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Each tube was then shaken at room temperature for 30 minutes prior to being centrifuged again at 13,500 rpm for 10 minutes
Not all peptides do the same thing
Methods Materials For tissue culture Dulbeccos modified Eagles medium (DMEM), RPMI-1640 medium, hygromycin-B, and Fluo-4 acetoxymethyl ester were used (Invitrogen Carlsbad, CA)
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In this study, we show that Nestin + MSCs were able to differentiate into the osteoblast lineage and deletion of the IGF1R in Nestin + MSCs impaired osteoblast differentiation and decreased trabecular bone formation
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