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Effect on cell viability was assessed 72 h after transfection with GSTO1 siRNA or siSCRAM by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and normalized to mock-transfected control (transfection reagent)
Most researchers report no noticeable potency issues at the 5-week mark, but conservative protocols suggest using the vial within 4 weeks and discarding any remainder
Boil this well till the water turns a brownish colour
Kim JH, Lee GY, Maeng HJ, Kim H, Bae JH, Kim KM, et al
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