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As described by Tielan Wei et al., 2022, the primary obstacle with oral glutathione is low bioavailability due to gastrointestinal degradation

To determine the effects of stimulating TCA cycle activity, we treated hepatocytes with dichloroacetate, which stimulates substrate entry into the TCA cycle by inhibiting PDKs (Constantin-Teodosiu, Simpson, & Greenhaff, 1999

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The reaction solution was prepared by mixing 1.5 mL of 0.05 M monosodium phosphate buffer, 0.3 mL of 0.1 mM EDTA, 0.3 mL of 750 M nitroblue tetrazolium chloride, 0.3 mL of 130 mM methionine, 0.3 mL of 20 M riboflavin, 0.28 mL of distilled water and 0.01 mL of 4% insoluble polyvinylpyrrolidone to measure the activity of the SOD enzyme

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Briefly, 96-well ELISA microplates were coated with HGFR/c-MET Fc chimera (50 ng/well), blocked with 1% BSA, 5% sucrose, and 0.05% sodium azide in PBS, and then incubated for 2 h at 37C with HGF samples