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The heart of the SPE technique is the sorbent material, which directly influences the selectivity, sorptive capacity, and the format or the configuration of the resultant SPE device [5]

But not every compound behaves like protein or caffeine

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[10] [11] It is important to check folate status alongside B12, but folate supplementation should not be started before excluding B12 deficiency, as this may precipitate neurological complications

Ensuring proper administration technique (IM deep into muscle, or IV slowly diluted) also helps minimize adverse effects like injection pain or vein irritation