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This minor change not only affects the protonation of the chromophore leading to pronounced stabilization of the anionic form of the chromophore (Elsliger et al., E from 280 mV for roGFP2 to 238 mV for roGFP2-iL is lower than the shift for roGFP1-iL which at 229 mV is 62 mV less negative than its parent roGFP1

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