A gradual step-down gives the researcher time to implement dietary and behavioral strategies at each lower dose level, building habits that can partially compensate for the loss of pharmacological support
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We look at their health, the effects of medicines, and if they might get better on their own
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Methods Animals Male Swiss mice at 12 weeks of age (N = 40) were divided into 5 groups (n = 8 each) (Table 1) and kept in the vivarium of the State University of Montes Claros (UNIMONTES), Minas Gerais (MG), under standard temperature [22 2C], air humidity between 60 5%, 12h light/dark cycle, low sound levels (below 40 dB) with a balanced diet (50.3% carbohydrates, 41.9% protein and 7.8% fat (2.18 kcal/g Purina-Labine ) and filtered tap water ad libitum [38]
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