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For F-actin quantification, at the end of experiment, cells were trypsinized than fixed with PBS/ formalin 3% then incubated with PBS/ Triton 0.1%/ TRITC-phallodin 1 g/mL (Sigma Aldrich) during 5 min

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The absolute reduction in liver fat was a little over 4%, which corresponded to a more than 31% relative reduction, the researchers reported

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In addition to the drug or placebo injection, participants received individual counselling sessions every 4 weeks to help them adhere to a reduced-calorie diet (500 calories deficit per day relative to their own starting calorie intake) and increased physical activity (150 minutes a week)