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Antibodies were diluted in 0.5% BSA, and after staining, coverslips were mounted with ProLong Gold Antifade Mountant containing DAPI (Invitrogen, Waltham, MA, USA, P36935), and imaging performed at the Microscopy Imaging Laboratory (MIL), University of Toronto, Canada, using an LSM 880 Elyra Super resolution confocal microscope (Zeiss)

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Eight weeks after surgery, the patient was taken out of his CAM walker