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The cells were lysed in 20 mM Tris pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.1% Triton-X, 5 mM DTT, and one protease inhibitor tablet (Roche) by ultrasonication

This ensures treatment works well and is safe for each patient

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A review in the Annals of the New York Academy of Sciences on Thymosin Beta-4 mechanisms in tissue repair and regeneration