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It is a profound, cellular-level energy crisis

The B12 Deficiency Itself People fail to realize that when patients suffer from B12 deficiency, very often the disease is only diagnosed at its end stage

This is normal and expected, especially early in treatment

The membrane was blocked in Intercept Blocking buffer (LI-COR, 927-60001) for 1 h at room temperature (RT) and incubated overnight at 4 C with antibodies against primary antibodies listed in Table S2

Its biosafety was supported by hemolysis below 5%, stable hematological parameters, and the absence of obvious damage in major organs on H&E staining [129]

Results: The distribution of qF-stages was 0: 13%