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Recent reviews and empirical studies have demonstrated that CRISPR/Cas, recombineering, and heterologous-expression toolkits enable the precise engineering of lactic acid bacteria and other probiotic chassis (Mugwanda et al., 2023

Bioactive peptides short amino acid sequences capable of modulating cellular behaviour have been shown in numerous in vitro studies to influence fibroblast activity, keratinocyte migration, and the synthesis of key extracellular matrix components including collagen types I and III, elastin, and glycosaminoglycans

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The meniscus is made of a dense extracellular matrix (collagen fibers + proteoglycans)

Structural optimization revealed that incorporating small hydrophobic groups, such as cyclopropylmethyl, at the N 7 -position enhanced potency, while a sulfonyl group at the N 2 -position and (4-fluorophenyl) amino at the C 3 -position improved metabolic stability (Figures 7A,B)

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