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Sample layout example: Lanes 1-6 for GSSG concentration measurement and lanes 7-12 for total glutathione concentration measurement Q How to measure a sample blank if the sample is colored A If the sample has coloration, the sample blank can be subtracted by either of the following two methods

Aitken RJ, Curry BJ (2011) Redox regulation of human sperm function: from the physiological control of sperm capacitation to the etiology of infertility and DNA damage in the germ line