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whilst B12 toxicity is rare, unnecessarily high doses offer no additional benefit Be aware of interactions: metformin and acid-suppressing drugs can reduce B12 levels
To evaluate growth on solid media, in all cases -with the exception of the metE mutant, which only grew in the presence of B12 and, therefore, plating with and without B12 was performed from liquid cultures supplemented with B12- the liquid cultures grown without B12 were plated in solid 7H10-ADC plates supplemented with or without AdoCbl (10 g/mL) and/or L-Met (25 g/mL)
High NNMT, as often seen in obesity, may be associated with marks favouring fat storage
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