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Through reducing Wnt/-catenin expression, qingshen granules, metformin, or shenkang injection (SKI) have been shown to lessen renal fibrosis (Wang et al., 2019
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Additionally, during the acridine orange (AO) reaction for a lysosome membrane permeability assay, the cells with indicated treatments were stained with 2 g/mL AO (Invitrogen) for 15 min at 37 C
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