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For lipid peroxidation level, Cells were treated with the fluorescent dye BODIPY 581/591 C11 (D3861, Thermofisher Scientific), which inserts into lipid membranes and allows for quantitative assessment of oxidized versus non-oxidized lipids by fluorescing green or red, respectively
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The OCR and ECAR values were obtained at baseline and after injections of Seahorse XF Mito Stress Test Kit (Agilent) reagents: oligomycin (1.5 M), FCCP (1.0 M), and antimycin/rotenone (0.5 M)
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Transcript abundance was quantified with Kallisto, and differentially expressed genes (DEGs) were identified using DESeq2
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