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The effect was practical and immediate: licensed compounding pharmacies could no longer prepare injectable GHK-Cu, pushing demand toward gray-market sources with zero quality control

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Plates were subsequently washed with 0.1% polyethylene sorbitan monolaurate (Tween20)-Tris-buffered saline (TTBS), immediately fixed with cold 3.7% paraformaldehyde in PBS, and re-treated with the blocking solution overnight at 4C