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In summary, time interval between blood withdrawal and freezing varied from 11 to 32 hours
Study designs that require repeated sampling across multiple tissue sites, for example, may lean on IGF-1 LR3's longer window to reduce the number of administrations needed, while designs isolating a single site of injury or load may prefer PEG-MGF specifically because its action stays contained rather than becoming systemic
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The ischemia-reperfusion procedure (45 min ischemia + 120 min reperfusion) and anesthesia protocol were adapted from previously validated models used in our earlier experimental studies and comparable literature 10,12,14
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