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The protein bands were visualized using the Pierce ECL Western Blotting Substrate (Thermo, 32106)

Chen PS, Toribara TY, Huber W (1956) Microdetermination of phosphorus

Vitamin C is essential for collagen synthesis, immune support, and overall health

Funding This work was supported by the National Natural Science Foundation of China (8247143079), The Discipline Construction Project of Hunan University of Chinese Medicine (Z2023JBGS05), the Key Project of Education Bureau of Hunan Province (23A0293), the Hunan Province Science and Technology Innovation Leading Talent Project (2021RC4034), the Key Project at Central Government Level: the ability establishment of sustainable use for valuable Chinese medicine resources (2060302)

Some herbs, including turmeric, rosemary, milk thistle, and ginkgo biloba have been shown to increase glutathione activity in the body

Interest in GHK-Cu has expanded from topical skincare and laboratory research into broader healthy-aging routines, although evidence depends strongly on the delivery route and product formulation