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(18) (18) Purification (17)BPC1571.23g13mlC 18 (Kromasil-C 18 20mm*250mm)ABA0.199.9B0.199.9A100B10010ml/min215nm()0.69g(MW1419.6)HPLC98 Dissolve 1.23 g of the crude BPC157 peptide in step (17) in 13 ml of water, filter, and load the filtrate on a C 18 chromatographic column (Kromasil-C 18 , 20mm*250mm, Hanbang Technology Chromatography Company), the mobile phase is as follows A, B Mixture of liquids: A: 0.1% trifluoroacetic acid plus 99.9% water